General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 P2RY8 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0635C2)
The PathHunter P2RY8 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. UTP) based activation of P2RY8 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY8 and EA-tagged β-Arrestin-2. Activation of P2RY8 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter PTGFR Total Internalization U2OS Cell Line, 2 vials. (93-0872C3)
The PathHunter PTGFR Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of PTGFR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGFR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGFR receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section - Human Bladder Tumor, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Multiple Species Paraffin Tissue Array - Liver, 5 slides/PK
BioChain's tissue arrays or tissue microarrays (TMAs) are a useful tool in biomarker research and drug target validation. It consists of paraffin blocks that are assembled in array fashion to allow multiplex histological analysis. BioChain's TMAs provide a valuable tool for analysis of protein, RNA, or DNA molecules in oncology, both the malignant and metastatic markers. TMAs can also be constructed with tissues from other diseases and species.
Eurofins Discoverx ChemiScreen TP Prostanoid Receptor Stable Cell Line, 2 vials. (HTS081C)
The ChemiScreen TP Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin H2, thromboxane A2) based activation of TBXA2R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TBXA2R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of TBXA2R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter AGTRL1 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0710C3)
The PathHunter AGTRL1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of AGTRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC 2E8; Bone Marrow B Lymphocyte; Mouse (Mus musculus)
2E8 is a B lymphocyte cell that was isolated from a mouse's bone marrow. The cells can be used to detect and quantify IL-7.
Abcam SNC80, delta opioid receptor agonist, 10MG
MW 449.64 g/mol, Purity >99%. Highly potent and selective δ opioid receptor agonist (Ki = 9.4 nM). Centrally active following systemic administration. Displays antidepressant activity.
The product is subject to the following: Abcam Restricted Use Statement
Abcam SKF 97541, Highly GABAB agonist, 10MG
MW 137.12 Da, Purity >98%. Highly potent GABAB agonist, 10-fold more potent than Baclofen (ab120149). (EC50 values for depression of striatal synaptic potentials are 920 nM and 1.25 μM for SKF97541 and baclofen, respectively). Also available in simple stock solutions (ab146688) - add 1 ml of water to get an exact, ready-to-use concentration.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx ChemiScreen PK1 Prokineticin Receptor Stable Cell Line, 2 vials. (HTS074C)
The ChemiScreen PK1 Prokineticin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PROKR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiBrite FFA1/GPR40 Free Fatty Acid Receptor Stable Cell Line, 2 vials. (HTS038L)
The ChemiBrite FFA1/GPR40 Free Fatty Acid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR1, a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FFAR1, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Promocell GmbH PromoCell HDMEC Pellet from > 1 million Normal Human Dermal Microvascular Endothelial Cells, adult
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Eurofins Discoverx ChemiScreen α2A Adrenergic Receptor Membrane Preparation, 1 vial. (HTS096M)
The ChemiScreen α2A Adrenergic Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRA2A surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
AllCells LLC AllCells Human Healthy Donor Peripheral Blood Leukopak, Fresh, 5 billion cells minimum
Leukapheresis is performed using the Spectra Optia Apheresis System. Mononucleated cell-rich blood is continuously collected using ACD-A as an anticoagulant. This product provides no less than 5 billion total nucleated cells (TNC). TNC in our leukapheresis products are composed of, on average, 50% T cells, 20% monocytes, 10% B cells, 7% NK cells, and 3% granulocytes. Actual product volume, TNC concentration, and cell type percentages vary from collection to collection.
HLA and CMV status are available upon request. All donors undergo an in-person screening including bloodborne pathogens testing, Hepatitis B, Hepatitis C, and HIV 1 and 2.
AllCells boasts the largest donor database in the industry. Our pool of healthy, diverse and recallable donors provides researchers with the right cells at the right time. AllCells donors undergo initial in-depth screening, followed by regular re-screening at three month intervals.
ATCC G-361; Melanoma; Human (Homo sapiens)
G-361 is a cell that has an epithelial morphology that was isolated from the skin of a White, 31-year-old, male with malignant melanoma. This cell line was deposited by PT Peebles and can be used in toxicology research.